Time 1 represents fish sampled as soon as unresponsive to external stimuli/less
than 90 s and time 2 represents fish sampled after 30min of entering the euthanasia. Challenge
salinity and euthanasia salinity were found to have a significant effect (α of 0.05) on
sodiumconcentration. Only time in euthanasia had a significant effect on cortisol concentration.
Challenge salinity, euthanasia salinity, and time in euthanasia had a significant effect
on osmolality