Leaves of Indian almond tree were collected from some area in Bangkok, Thailand.
The leaves were dried in laboratory room at room temperature. To yield 50 mg/ml concentration,
1 kilogram of dried leaves was minced and soaked in 20 liters of distilled water for 1, 3, and
7 days at room temperature. The extracted solution was filtered (Whatman paper No. 4). Then,
various dilutions of the extract were prepared for optical density (OD) measurement by using
Spectrophotometer at 245 nm. wave length. Antibacterial activity and toxicity test were
determined by using the extract for 3 days