The isolation of LAB from Plasom at various fermentation periods was performed. MRS agar containing 0.3% (w/v) CaCO3 was used as a preliminary screening medium for LAB. It was found that 138 isolates exhibited a clear zone and growth on MRS agar supplemented with CaCO3. Calcium carbonatewas used as an indicator for acid-producing strains since it dissolved when interact with acid then a clear zone is observed (Onda et al., 2002). However, 133 isolates were identified as LAB using the criteria of bein tive. Of these 133 isolates, 25 isolates were cocci; 75 isolates were short rods and 33 isolates were rods
(data not shown).