Human have the microorganisms in the skin, gut and mouth. Human skin microbiome based on the 16S rRNA
encoding gene can reveal bacterial species diversity. Also bacterial species in the skin have diverse and unique
composition between individuals. We thought that a bacterial fingerprint obtained from surfaces including computer
keyboards aids forensic individual identification in case of evidence deficiency. Next generation sequencing was
used to analyze the bacterial community on objects and fingertips to match the object to the individual. The 16S
rRNA gene sequence was submitted to EMBL SRA with accession number PRJEB8760. Higher similarity of
bacterial community between public computer keyboards and laboratory member’s fingertips were evident than
between other locations including doorknobs. Here we studied the challenges that bacterial fingerprint can be used
as a human identification tool in forensic fields.