Bi-layer tablets were prepared in our laboratory by
compressing 50 mg of ATL and 10 mg ATV. Twenty tablets
were taken and were crushed to a fine powder. The powder
sample equivalent to 50 mg of ATL and 10 mg of ATV was
transferred to a 10 mL volumetric flask and about 80 mL of
50% v/v aqueous methanol was added and sonicated to
dissolve. The volume was made up to the mark with 50% v/v
aqueous methanol. This solution was filtered through
Whatman filter paper. 42.1 mL of this solution was diluted to 100 mL with 50% v/v aqueous methanol. The solutions were
analyzed by multicomponent mode of analysis. The samples
were analyzed six times. 50% v/v aqueous methanol was
used as blank. Fig.1 shows overlay spectrum of ATL and
ATV.