In the AI-NS group, the 25th percentile days to pregnancy for heifers with RTS 3 and 4 both had a median time
of 10 days. Therefore, we infer that the heifers with RTS 3 were closer to onset of puberty than expected, or that our
distinction between RTS 3 and 4 was not accurate. It is plausible that the synchronization protocol with CIDR
might have helped heifers with RTS 3 or less to achieve puberty. Furthermore, no difference was noted for AI-NS
group for 75th percentile days to pregnancy for heifers with RTS 3 and 4 (50 and 50 days, respectively) substantiating
that this group of heifers benefited from synchronization protocol.