Mycelium bound lipase of A. niger NCIM
1207 produced maximal isoamyl acetate formation at an
alcohol/acid ratio of 1.6. Acetic acid at higher concentrations
than required for the critical alcohol/acid ratio lower
than 1.3 and higher than 1.6 resulted in decreased yields of
isoamyl acetate probably owing to lowering of microaqueous
environmental pH around the enzyme leading to
inhibition of enzyme activity.