We have evaluated methods in which glucose oxidase
is used to determine serum glucose with the
“AutoAnalyzer-Il”and “SMA 12/60” (Technicon). A
glucose oxidase-peroxidase-dianisidine procedure
with improved specificity developed during this study
is described. The glucose value isunaffectedby the
presence of 16 mg of uric acid per deciliter, and the
system is highly resistant to interference from many
other reducing compounds added to serum. The
method appears to give “true glucose” values for
normal, hyperuricemic, and uremic subjects. Results
are compared with those from the “GOD-Perid,” glucose
oxidase-peroxidase-M BTH-DM A, hexokinase,
toluidine, ferricyanide, and SMA 12/60 neocuproine
techniques, as well as with those from our index of
“trueglucose,”a procedure based on measurement
of reducing materialbefore and aftertreatmentwith
glucose oxidase. Specificity arid performance of the
glucose oxidase-peroxidase-dianisidine and glucose
oxidase-peroxidase-M BTH-DM A procedures are
similar.