Acrosome integrity
Sperm acrosome status was assessed by lectin PNA from Arachis hypogaea Alexa Fluor® 488 conjugate. Diluted semen samples were mixed with 10µL of PNA working solution (1µg/mL) and incubated for 5 min in room temperature in dark. After incubation, the samples were washed and 5µL of PI were added before cytometric analysis[34].