Kim et al. studied the isotope effects of uranyl complexes by means of ion exchange chromatography and reported that the malic acid eluent system had the largest separation coefficient among some selected uranyl carboxylate complexes [23]. Therefore, the purpose of the work is to study the isotope effects of neodymium in ligand exchange system using glycolic, malic and citric acids as mono, di and tri carboxylic acid to compare the effect of different ligands on the isotope effects of Nd in Nd-Ligand exchange system. It is aimed to find the most suitable ligand that gives the highest separation coefficient and to get more information that may lead to more understanding of the theory of isotope effects.