The complete absence of autochthonous microbiota was
assessed on uninoculated reference samples before and after each
pressure treatment with a detection limit of 2 Log cfu/mL. Aseptically
neutralized sample (100 mL) was inoculated in TSBYE (1 L)
and incubated at 30 C for 48 h. The enriched culture was plated on
Dichloran Rose Bengal Chloramphenicol Agar (DRBC) (EN ISO
21527) for yeast and moulds, on Selective ALOA Listeria agar (ISO
11290-1 Amd1:2004), for Listeria spp. and on PCA for the total
aerobic mesophilic counts (UNI EN ISO 4833).