Although circulating neoplastic myeloid cells
(myeloblasts) could be misclassified as circulating
neoplastic lymphocytes, it is unlikely that cells identified
as circulating large lymphocytes were myeloid in
origin, given the previous diagnosis of lymphoma in
the dogs included in this study and the presence of a
mature circulating neutrophil population. Likewise,
the differentiation between neoplastic lymphocytes
and myeloid precursors can be difficult in bone marrow
smears. However, the myeloid series in the
examined bone marrow smears demonstrated orderly
maturation, and was distinguished from neoplastic
lymphoid cells based on morphologic characteristics
including lower nuclear to cytoplasmic ratio and the
presence of primary or secondary granules. Additional
tests to confirm the identity of the cell populations
might include antibody-mediated phenotyping
by flow cytometry, immunocytochemistry, or immunohistochemistry.
As small-cell lymphoma cannot be
reliably diagnosed based on cell morphology, dogs
with small-cell lymphoma were not included in this
study.
Although circulating neoplastic myeloid cells(myeloblasts) could be misclassified as circulatingneoplastic lymphocytes, it is unlikely that cells identifiedas circulating large lymphocytes were myeloid inorigin, given the previous diagnosis of lymphoma inthe dogs included in this study and the presence of amature circulating neutrophil population. Likewise,the differentiation between neoplastic lymphocytesand myeloid precursors can be difficult in bone marrowsmears. However, the myeloid series in theexamined bone marrow smears demonstrated orderlymaturation, and was distinguished from neoplasticlymphoid cells based on morphologic characteristicsincluding lower nuclear to cytoplasmic ratio and thepresence of primary or secondary granules. Additionaltests to confirm the identity of the cell populationsmight include antibody-mediated phenotypingby flow cytometry, immunocytochemistry, or immunohistochemistry.As small-cell lymphoma cannot bereliably diagnosed based on cell morphology, dogswith small-cell lymphoma were not included in thisstudy.
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