Bull and semen treatment order were randomized and assigned before breeding based on
the order in which females exhibited estrus or chute order at timed AI. Inseminations were preassigned
in this order to account for different numbers of straws available from each sperm
sorting treatment so that an approximately equal distribution of estrus-AI and timed AI
inseminations occurred with each bull and sperm treatment (n = 240, 228, 170 and 160 for 20M
CON, 10M CON, 10M PNA, and 10M UBI, respectively).