This study was conducted to isolate psychrotrophic lactic acid bacteria (LAB) from chicken carcasses with
inhibitory activity against strains of Salmonella spp. and Listeria monocytogenes. A total of 100 broiler
samples were examined for the presence of LAB. Ninety-two LAB isolates that showed antimicrobial
effects against Salmonella spp. and L. monocytogenes were further analysed to examine their LAB (Grampositive,
catalase negative, oxidase negative) and psychrotrophic characteristics (ability to grow at 7
C).
Fifty isolates were further selected and identified initially using standard biochemical tests in miniature
(Micro-kits API CH 50) and then by sequencing of the 16se23s rRNA gene boundary region (Intergenic
Spacer Region). By molecular identification, these isolates were classified into 5 different LAB species:
Lactobacillus salivarius, Lactobacillus reuteri, Lactobacillus johnsonii, Pediococcus acidilactici, and Lactobacillus
paralimentarius. None of the isolates produced tyramine or histamine.