bacterial growth medium broth then were anaerobically incubated using BBL gas packs at 37°C for 16 hours. Fluid
milk was then inoculated with a 1% by weight sample of the probiotic broth mixture and 0.4% yeast extract. The inoculated milk was then incubated at 37°C for 18 hours then stored in an airtight container before transfer to the yogurt manufacturing site.