3.3. Validation of the method used for plant sterol analysis
The GC method was able to separate 12 plant sterols and cholesterol individually and two sterols coeluting as one peak in 30 min. As calibration functions were similar for the five investigated sterols with response factors close to 1 as found also by Dutta and Norme ´ n (1998) and Laakso (2005), the parameters determined for