The primers successfully amplified targeted DNA fragments for each PCN region. Paired primers of ITS-1 and 5,8S rDNA produced 238 bp DNA fragment for all regions (West Java, East Java, and Central Java) (Fig. 1),
while ITS-1 and 5,8S rDNA resulted in a 391 bp DNA fragment only for the Central Java isolates (Fig. 2). According to Mulholland et al. (1996) the fragments of 238 bp and 391 bp are related to G. rostochiensis and G. pallida, respectively.