3.6. Extracts and fractions
As shown in Table 1, the three methanolic fractions
obtained by diethyl ether, ethyl acetate and n-butanol
had the highest total phenol content and exhibited the
best radical scavenging capacity among the others (Tables
2 and 3). The ether fraction exhibited the second
highest AE values in both RSA tests after the ethyl acetate
fraction, but in the TPC test, exhibited the third
highest value. The second highest value in TPC test after
the ethyl acetate fraction was possessed by the butanol
fraction, which exhibited the third highest AE values
in both RSA tests. Dichloromethane extract exhibited
the fourth highest TPC and AE value in both RSA tests.
3.7. Extracts/fractions and reference antioxidants
Three different standards known for their good antioxidant
activity, trolox, ascorbic acid and quercetin
were used. These standards were suggested by Parejo
et al. (2000) who used them to evaluate scavenging activity
by luminol chemiluminescence and DPPH assays.
The radical scavenging activity of the standards increased
in the following order: Ascorbic acid < Trolox <
Quercetin.
Trolox was found to be 7.2 times more active than
ethyl acetate fraction, ascorbic acid was 9.1 times more
active and quercetin 10.9 times more active. Further,
trolox was found to be 9.9 times more active than the
ether fraction, ascorbic acid 12.5 times and quercetin
3.6. Extracts and fractions
As shown in Table 1, the three methanolic fractions
obtained by diethyl ether, ethyl acetate and n-butanol
had the highest total phenol content and exhibited the
best radical scavenging capacity among the others (Tables
2 and 3). The ether fraction exhibited the second
highest AE values in both RSA tests after the ethyl acetate
fraction, but in the TPC test, exhibited the third
highest value. The second highest value in TPC test after
the ethyl acetate fraction was possessed by the butanol
fraction, which exhibited the third highest AE values
in both RSA tests. Dichloromethane extract exhibited
the fourth highest TPC and AE value in both RSA tests.
3.7. Extracts/fractions and reference antioxidants
Three different standards known for their good antioxidant
activity, trolox, ascorbic acid and quercetin
were used. These standards were suggested by Parejo
et al. (2000) who used them to evaluate scavenging activity
by luminol chemiluminescence and DPPH assays.
The radical scavenging activity of the standards increased
in the following order: Ascorbic acid < Trolox <
Quercetin.
Trolox was found to be 7.2 times more active than
ethyl acetate fraction, ascorbic acid was 9.1 times more
active and quercetin 10.9 times more active. Further,
trolox was found to be 9.9 times more active than the
ether fraction, ascorbic acid 12.5 times and quercetin
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