SG is a common components that usually found in Acrylated Sterol Glucosides (ASG) form. During transesterification process, ASG can be converted to SG which their polarity was changed and restrictive solubility in biodiesel. When biodiesel has a high concentration of SG, that was formed to white precipitate in room temperature consistent with results from Fourier Transform Infrared Spectrometer (FT-IR) spectra show ASG and SG are major components of precipitate.