The member of PHA family, (3HB-co-3HV) copolymer, is one of
the best characterized because of its high commercial potential.
Some microorganisms were capable to biosynthesize P(3HB-co-
3HV) copolymers from various crude plant oils, however it was
dependent on the type of 3HV precursors used. Lee and coinvestigators
(2008) demonstrated the production of P(3HB-co-
3HV) copolymer by Cupriavidus necator H16 with 3HV molar fraction
ranging from 3 to 14 mol% by adding sodium valerate and
sodium propionate as the incorporators of 3HV monomers. When a
mixture of palm kernel oil and sodium propionate was used the
copolymer content (90% of CDW) and biomass value (7.5 g/L) were
the highest with 3HV fraction of only 7 mol%. Whereas a combination
of olive oil and sodium valerate resulted in the highest 3HV
monomer composition (14 mol%) with a copolymer content of 89%
of CDW. Also, waste rapeseed oil was found to be an excellent
substrate for the growth and P(3HB-co-3HV) production by
Cupriavidus necator H16. Obruca et al. (2010) enhanced copolymer
production by adding precursors such as propanol, propionate and
valerate. Propanol improved both biopolymer and biomass formation,
up to 80% of CDW and 14.7 g/L of biomass was obtained.
The highest 3HV molar fraction of 18 mol% was obtained through
the feeding of valeric acid.
The member of PHA family, (3HB-co-3HV) copolymer, is one ofthe best characterized because of its high commercial potential.Some microorganisms were capable to biosynthesize P(3HB-co-3HV) copolymers from various crude plant oils, however it wasdependent on the type of 3HV precursors used. Lee and coinvestigators(2008) demonstrated the production of P(3HB-co-3HV) copolymer by Cupriavidus necator H16 with 3HV molar fractionranging from 3 to 14 mol% by adding sodium valerate andsodium propionate as the incorporators of 3HV monomers. When amixture of palm kernel oil and sodium propionate was used thecopolymer content (90% of CDW) and biomass value (7.5 g/L) werethe highest with 3HV fraction of only 7 mol%. Whereas a combinationof olive oil and sodium valerate resulted in the highest 3HVmonomer composition (14 mol%) with a copolymer content of 89%of CDW. Also, waste rapeseed oil was found to be an excellentsubstrate for the growth and P(3HB-co-3HV) production byCupriavidus necator H16. Obruca et al. (2010) enhanced copolymerproduction by adding precursors such as propanol, propionate andvalerate. Propanol improved both biopolymer and biomass formation,up to 80% of CDW and 14.7 g/L of biomass was obtained.The highest 3HV molar fraction of 18 mol% was obtained throughthe feeding of valeric acid.
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