For enzymatic hydrolysis, the pretreated RSM slurry was adjusted to pH 6.0 using
40% NaOH. The enzymes, Pectinase, Celluclast or Viscozyme or their mixtures, were
added to the pretreated RSM at a total enzyme loading of 2% (w/w, on dry matter).
Beta-glucosidase was added at 20 U/g dry matter. The enzymatic hydrolysis was
carried out at 38 ◦C for 48 h.