The leaf litter segment was comminuted using sterile water and a scalpel, placed on a glass slide, stained with lactophenol and observed under a bright field microscope (400, Nikon, Labophot 2) for the presence of fungal spores.
The leaf litter segment was comminutedusing sterile water and a scalpel, placed on a glass slide,stained with lactophenol and observed under a bright fieldmicroscope (400, Nikon, Labophot 2) for the presence offungal spores.