Precultures were prepared from solid agar medium and
incubated for overnight at 30 °C and 180 rpm. Studies on
PHA production were conducted in sterilized 250 mL
Erlenmeyer flasks with 50 mL of mineral culture medium for
the others substrates, inoculated with 1 mL of bacteria from a
seed culture and incubated at 30 °C with shaking at 180 rpm.
The fatty acids were added as sole carbon source in each test
flask according to the text above