TM1, one of the muscle fiber type-related proteins, was
observed as a commonly distributed protein in all species. However, LC-MS/MS spectra allow the identification each species from
fresh meat and meat mixtures. Another protein, CA3, which is distributed in type I (slow-twitch) fibers was observed in bovine and
porcine longissimus muscles, which contain type I fibers. Proteins
identified as TnI, enolase 3, LDH and TPI were found in all samples
and could be markers for the discrimination of mammals from
poultry. Furthermore, species-specific spectra from these proteins
can be peptide markers for the identification of each species following additional analysis by LC-MS/MS. Therefore, discrimination
of meat species from fresh meat and their mixtures is possible by
comparing the electrophoretic mobility and through immunoblotting and LC-MS/MS analysis, and we confirmed that commonly distributed proteins in all species can also be candidates for meat
discrimination.