In this experiment we can determine the presence of coliform bacteria in a water sample that it's E. coli. by transfer in water sample containing lactose lactose broth incubate at Durham with 12 hr 37 tubes.After we will get positive result in fermentation, gas tube, gas tube, and no fermentation in result.Next we are negative EMB agar plate from streak tube according to the number of positive tubes resulting plate incubate at Later gas. 12hr E.coli can be we will get 37 product dark metallic sheen on EMB agar and agreen.