Both spent coffee extracts showed slightly greater amounts of all the CQAs and diCQAs than their respective coffee brews, with the difference (p < 0.05) in Robusta samples being quite significant. Arabica spent coffee extract had a final concentration of total chlorogenic acids (CQA and diCQA) of 84.22 mg/g, whereas Robusta spent coffee extract had 66.09 mg/g.