With the continuing expansion in genomic datasets of bacteria it
has been possible to identify an increasingly larger number of
microorganisms. Utilization of MALDI TOFeTOF MSeMS sequencing
of ions generated from tryptic digests of proteins provides the
peptide sequence for comparison. This approach does not require
any previous knowledge of the bacterial species. In this paper, tryptic
peptides analyzed by MALDI TOF MS profiles allowed the characterization
of the species identity of environmental isolates. MALDI
TOFeTOF MSeMSwas used to identify proteins from these peptides.
Protein identification definitively categorized Micrococcus from
environmental isolates and also distinguished them from staphylococcal
species isolated from the same environment.