Capture techniques, such as the use of seines and traps, gill netting, ichthyocides, electrofishing, and hooks or spears must be carefully reviewed as to their necessity and the possibility of capture distress (ASIH 1987). The fish habitat should be as undisturbed as possible to support the concept of habitat conservation. Restraint and handling in the particular proposal should look to limit the amount of time of handling, especially with large or venomous fish. A team approach when working with these fish should be encouraged or required depending on the particular circumstances. In any case, the investigator (or investigators) should be experienced in handling these species or have a plan to receive training prior to the initiation of the protocol. In general, a protocol should not be approved by the IACUC unless the investigator has appropriate training in the particular handling, care, and use of the species proposed. Prolonged restraint should be avoided (see above, under Anesthesia, Analgesia, and Euthanasia).