Over one year, 80 OPD patients with corneal ulcers were scraped from the margins & base of the anaesthetized cornea & smear prepared for Gram staining & 10% KOH preparation. Blood agar for both aerobic & anaerobic, Mac-Conkey Agar, Chocolate Agar were inoculated. Turbidity in brain heart infusion were identified by Gram Stain & subsequently sub-cultured in Mac-Conkey & blood agar. Anaerobic jar containing the blood agar plates were incubated at 37oC & examined after 48-72 hrs, & finally for 5 days & examined on alternative days before discarding. Further microbiological identification done as per standard protocol. All the isolated bacteria tested against different antimicrobial agents by standard disc diffusion method in accordance with CLSI guideline 2012. Out of 80 cases, corneal ulcer showed male preponderance (3:1) the highest no.19 (23.75%) cases were in the age group of 51-60 years. A total of 40 positive cultures were aerobes.12 specimens for anaerobic culture showed no growth. Among the isolates 19 (46.34%) were Staphylococcus aureus, 13 (31.7%) were CONS, 5 (12.19%) were Pseudomonas, 3 (7.31%) were E. Coli & 1 (2.43%) was Klebsiella. Gram positive cocci were maximally sensitive to Vancomycin, Tobramycin with highest resistance to Ciprofloxacin (55.87% sensitive). Gram negative isolates were maximally sensitive to Chloramphenicol & Moxifloxacin & resistant to Norfloxacin (44.44% sensitive). Routine microbiological examination of corneal ulcer is necessary to analyse & compare the changing trends in the microbial etiology
& their susceptibility pattern to formulate a proper & appropriate antibiotic response against corneal ulcer.