The addition of 10 % (w/v) AC into the medium, however, significantly improved embryo germination and rooting. The use of two root promoting PGRs (IBA and NAA) were also evaluated. We noted that both the PGRs enhanced embryo germination, but 1.0 mg l-1 IBA was more active compared to the higher concentration (2.0 mg l-1), this effect was even more enhanced in AC supplemented medium (Table 3), where maximum rooting (81.99 %) was observed (Fig. 1F).
The addition of GA3 and ABA also showed similar promotive effect and use of AC into this medium improved germination further, maximum being on 1.0 mg l-1 in both cases.