2.3.1. CCWP(1)
Aqueous methanol (80%, 500 ml) was added to defatted VOR (100 g). The contents were boiled for 1 h under reflux. The insoluble matter was collected by filtering through double layers of a linen cloth and was mixed with 1% sodium dodecylsulphate (SDS) and 1% mercapto ethanol mixture (500 ml) at room temperature for 1 h with occasional stirring. The contents were filtered through double layers of linen cloth to collect insoluble matter and were washed with distilled water three times. The insoluble matter collected in the above steps was added to 90% DMSO (400 ml) and mixed for 1 h. The solvent was filtered through double layers of a linen cloth and the cell wall polysaccharides were collected by washing three times with distilled water and dried at 80 °C to obtain CCWP(1)..