100μl
portions of diluted extracts were introduced into test tubes
followed by addition of 750μl of freshFolin-Ciocalteu
reagent (1:10). After standing at room temperature for 5 min,
750μl of 6% (w/v) sodium carbonate (Na2CO3) was added
and allowed to completely reacted for 90 min at the room
temperature in the dark condition. And then, the absorbance
ofthe reaction mixtures were recorded at 725 nm against
blank. Total phenolics were calculated from standard
gallic acid solutions (0.02 - 0.1 mg/ml) used under the same
conditions, and concentrations were expressed as mg gallic
acid equivalent per gramdry weight (mg GAE/g DW).