With regard to the extraction solvent, and given that PAH
metabolites present high solubility in acetonitrile, this solvent
was chosen. Because enzymatic hydrolysis in the following step
is active in aqueous media, the extraction organic solvent must
be evaporated to dryness. However, the temperature applied
during the evaporation step may limit the recoveries of
hydroxylated metabolites. Different temperatures were tested
(35, 37, 40, and 42 °C) and, in agreement with other studies,17
the highest temperatures degrade these compounds and,
therefore, 37 °C was selected. In addition, to decrease the
time of the evaporation step, two volumes of extraction solvent
were tested, 6 and 10 mL. Nonetheless, the best recovery
results were attained with 6 mL because a reduction in the
evaporation time was accomplished. It is worth noting the
importance of avoiding light and maintaining a low temperature
to minimize metabolite degradation. Good separation between
the sample and supernatant
With regard to the extraction solvent, and given that PAH
metabolites present high solubility in acetonitrile, this solvent
was chosen. Because enzymatic hydrolysis in the following step
is active in aqueous media, the extraction organic solvent must
be evaporated to dryness. However, the temperature applied
during the evaporation step may limit the recoveries of
hydroxylated metabolites. Different temperatures were tested
(35, 37, 40, and 42 °C) and, in agreement with other studies,17
the highest temperatures degrade these compounds and,
therefore, 37 °C was selected. In addition, to decrease the
time of the evaporation step, two volumes of extraction solvent
were tested, 6 and 10 mL. Nonetheless, the best recovery
results were attained with 6 mL because a reduction in the
evaporation time was accomplished. It is worth noting the
importance of avoiding light and maintaining a low temperature
to minimize metabolite degradation. Good separation between
the sample and supernatant
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