4. Conclusions
It is clear from this study and other published works that low specific growth rate control feeding consistently helped to enhance the production of extracellular microbial lipase in HCDF.This is irrespective of the nature of the immiscible substrate used for growth and lipase production i.e., either a pure fatty acid such as oleic acid or whole plant oil such as palm oil. The exact reason behind the increased lipase production when this specific feeding mode was used in HCDF is unclear at this stage. It is suggested that a more detailed study using tools such as proteomics analysis may provide better explanation for the observed effect.