From the EHP-infected penaeid shrimp, the hepatopancreas was
removed and pooled (4–5 shrimp) into one sample. From tanks
holding EHP-infected shrimp, feces and water were sampled. The
tank water was concentrated 150 times with a Microsep Advance
Centrifugal Device (PALL Corporation) and used for DNA extraction.
Total DNA was extracted from each sample using a High-pure DNA
template preparation kit (Roche Bioscience) or a Maxwell-16 Cell
LEV DNA purification kit (Promega).