4. Discussion
Neem is an indigenous herb which was chosen for this study. It
belongs to the family of Meliaceae. The lack of information and
medicinal importance on this plant facilitate this kind of study. In
vitro total phenolics and flavonoids contents were determined for the
crude extracts from the stems of neem. The total phenolics content
of the crude extracts from the stems was expressed as gallic acid
equivalents (GA) per gram of dry extract ranged between 12.18 to
128.5 mg GA/g. The results showed that the highest amount of the
total phenolics was in ethyl acetate and the lowest was in hexane.
The order was ethyl acetate > chloroform > butanol > methanol >
hexane. The gallic acid was used as a standard calibration curve.