Methodology and Results: Four different pathogenic bacteria species viz., Shigella dysenteriae, Streptococcus lactis,
Bacillus cereus and Klebsiella pneumoniae were isolated which showed a wide range of sensitivity to norfloxacin,
tetracycline, ciprofloxacin, ofloxacin and nitrofurantoin. S. dysenteriae was sensitive to streptomycin where as other
isolates were found to be resistant. Agarose gel electrophoresis failed to reveal plasmid DNA band indicating that the
observed resistance was perhaps encoded by nucleotide sequences harboured on the chromosomal DNA. Bacterial
isolates were used as antigen for the production of polyclonal antibodies in rabbits.
Methodology and Results: Four different pathogenic bacteria species viz., Shigella dysenteriae, Streptococcus lactis,Bacillus cereus and Klebsiella pneumoniae were isolated which showed a wide range of sensitivity to norfloxacin,tetracycline, ciprofloxacin, ofloxacin and nitrofurantoin. S. dysenteriae was sensitive to streptomycin where as otherisolates were found to be resistant. Agarose gel electrophoresis failed to reveal plasmid DNA band indicating that theobserved resistance was perhaps encoded by nucleotide sequences harboured on the chromosomal DNA. Bacterialisolates were used as antigen for the production of polyclonal antibodies in rabbits.
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