Sub- and super-critical extraction conditions affected, to a considerable extent, the fat-soluble pigment content of the extracts. Analysis of pigments by HPLC provided separation of the individual carotenoid and chlorophyll constituents of the
extracts. Among carotenoids lutein as minor and -carotene as major compounds could be detected. Our focus was only on
-carotene due to its important role as bio-antioxidant and vitamin A precursor [18]. The HPLC profile consisted of different chlorophyll and Mg-free derivatives (pheophytins). The sum of chlorophyll a and b and that of pheophytin a and b was in the
evaluation of the quality attributes of the extracts.