2. Materials and methods
2.1. Microalgae biomass
Three different microalgae strains were used for this study: two
marine species, N. gaditana and P. tricornutum, and a freshwater species,
C. sorokiniana. These species are potentially good candidates for biofuel
production since they have high photoautotrophic biomass and lipid
production rates and can grow to high densities (N10 g L−1) while
tolerating a wide range of conditions with regard to pH and temperature
[22,23].
The microalga N. gaditana was obtained as a paste, containing 78%
(w/w, wet basis) moisture, whereas the microalgae C. sorokiniana and
P. tricornutum were in lyophilized state. For the microalgae powders,
distilled water was added before its use, to reproduce the harvested
and concentrated microalgae with water content of 80–90%.
Table 1 shows the elemental analysis (C, H, S, N and O contents)
of the microalgae, which was determined using a Leco 628 Series
Analyser.
2. Materials and methods
2.1. Microalgae biomass
Three different microalgae strains were used for this study: two
marine species, N. gaditana and P. tricornutum, and a freshwater species,
C. sorokiniana. These species are potentially good candidates for biofuel
production since they have high photoautotrophic biomass and lipid
production rates and can grow to high densities (N10 g L−1) while
tolerating a wide range of conditions with regard to pH and temperature
[22,23].
The microalga N. gaditana was obtained as a paste, containing 78%
(w/w, wet basis) moisture, whereas the microalgae C. sorokiniana and
P. tricornutum were in lyophilized state. For the microalgae powders,
distilled water was added before its use, to reproduce the harvested
and concentrated microalgae with water content of 80–90%.
Table 1 shows the elemental analysis (C, H, S, N and O contents)
of the microalgae, which was determined using a Leco 628 Series
Analyser.
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