Burnett and Towle (3) have reviewed studies of the excised,
perfused gills at Carcinw maems and Eriocheir sinensis.
They note the many difficulties inherent in this
preparation, not the least of which is the need to prevent
gill tissue swelling by including glutathione and several
other organic solutes in the perfusion medium to maintain
a constant perfusion pressure. Further, they note that most
studies with perfused gills have used the same medium on