Median temperatures
24 h prior to sample collection were used to determine recent temperature
history for days 1, 7, 14 and 21. Treatment-aquaria were randomly
assigned among four fiberglass mesocosm tanks, which were flushed
with seawater from adjacent Masonboro Sound to regulate temperature.
Mesocosms were placed on an outdoor platform arranged in an east–
west orientation to reduce shading by mesocosm walls.