UV–Vis spectrophotometry detection and acetone as the extraction medium was successfully employed for the simple and rapid determination of β-carotene content in four different types of tissue samples. In comparison to the saponification methods mentioned previously, the proposed UV–Vis spectrophotometry method is environmentally friendly and inexpensive and easily performed. In addition, simultaneous analysis was accomplished with high precision (repeatability and reproducibility). The method has great potential for adoption by many developing countries where limited funds are available for routine monitoring of such type of compound. Therefore the proposed method could be useful and practical in the future for β- carotene detection from various tissue samples.