The position of teeth was a significant factor for sealant retention. This result was consistent with previous 12.13. Application studies of this factor should be included and increased attention and awareness on the part of the provider when applying the sealant must be insured. The presence of an assistant was also a significant factor. However, a shortage of manpower is a normal occurrence for public health situations. Even though the ideal sealant application with a dental assistant was known by dental professionals, the shortage of resources and the hospital director's policy emphasis on in service treatment were big problems 
In cases of unfilled sealant, an occlusion check might be unnecessary20. However, in the context of this study, the relationship between the retention and an occlusion check gave a higher full retention rate than a retention check only. Therefore, both checks of sealant retention and occlusion after sealing are suggested.