supplemented with
various concentrations of 6-benzylaminopurine (BAP) and Kinetin
(Kin) (ranging from 1.0 to 6.0 mg l−1), and indol-3 acetic acid, IAA
(0.1 to 1.0mg l−1) were used alone or in combination to induce shoots
from the nodal meristems. Ten ml of medium (10 replicates) was
poured in each culture tube. All the experiments were repeated thrice.
Sterilized explants inoculated vertically on themediumand the cultures
were incubated in culture shelveswith illumination from white fluorescent
tubes at incubation temperature of 25 ± 2 °C to 30 ± 2 °C under
different light:dark photoregimes (12:12 h, 14:10 h, 10:14 h) for
shoot bud induction.