not out of the central ERC. It is not known if these effects are
limited to the trafficking of Tf/TfR or the compartments through
which Tf/TfR recycle.
To determine if cytoplasmic PLA2 enzymes have a more general
role in endocytic trafficking, we have examined the effects of PLA2
antagonists on the trafficking of various ligands and receptors
through the recycling and degradative endocytic pathways. We
find that the cytoplasmic PLA2 antagonists ONO and BEL inhibit
the export of soluble and membrane-bound cargo from various
endocytic compartments, in addition to inhibiting tubule formation
from endosomes containing these cargos. These results provide
evidence that cytoplasmic PLA2 activity is important for the
trafficking of multiple soluble and membrane-bound cargos