Tissue culture has been used for diverse purposes, being the regeneration of explants, during in vitro establishment, a decisive stage to development of this technique. One of the difficulties encountered in the initial stage, besides contaminations by microorganisms, is the regeneration of the excised explants, which normally requires exogenous source of plant growth regulators. The aim of this research was to evaluate the effect of plant growth regulators on the regeneration of Lavandula dentata shoot tips. Shoot tips were excised and placed on LS culture medium containing 20 g L-1 sucrose, 2.5 µM indole-3-butyric acid (IBA) and 6 g L-1 agar. Different 6-benzylaminopurine (BAP) concentrations with or without 0.3 µM gibberellic acid (GA3) were tested. The experimental design used was random blocks with four replicates of 10 explants per plot. The largest height of shoots was 2.7 cm obtained on medium containing 0.5 µM BAP + 2.5 µM IBA + 0.3 µM GA3. The highest number of leaves per shoot (± 8) was recorded on medium containing 1.0 µM BAP + 2.5 µM IBA and higher percentage of regenerated explants (90%) on medium containing 10.0 µM BAP + 2.5 µM IBA + 0.3 µM GA3. Plant growth regulators combinations promote initial development of the shoot tips, and the presence of GA3 favor the shoot elongation. Key words: Micropropagation, in vitro establishment, explants, aromatic plants