Sequence analysis of the BPH26 gene
Comparison of the cDNA and genomic DNA sequences revealed that there are three exons and two introns (Fig. 1c). The deduced open reading frame (ORF) of BPH26 encoded a protein of 1,218 amino acids. The cDNA and amino acid sequences are shown in Supplementary Fig. S3 online and Fig. 3, respectively. Motifs present in the protein encoded by BPH26 were assessed on the basis of the report of Zhou et al29. BPH26 also contained many kinds of motifs, e.g., CC motif; motifs characteristic of the NBS domain; and LRR motifs, which are found in other CC-NBS-LRR proteins (Fig. 3).