Solutions
were mixed and allowed to stand at room temperature for
2 h. Sample aliquots were filtered through a Whatman 0.45 m poly
(tetrafluoroethylene) filter prior to the determination of total phenolics
concentration using a UV spectrophotometer monitoring
640 nm. Total phenolics content was standardized against ferulic
acid and expressed as g/ml of ferulic acid equivalents (FAE). The
linearity range for this assay was determined as 0.5–5.0 mg/L FAE
(R2 = 0.9990), giving an absorbance range of 0.050–0.555 AU