Semi-scale production of PHAs
The successful production of PHAs in both shaken
flasks (Gamal et al., 2011) and 3 L laboratory fermentor
(Gamal et al., 2012) cultures generate the trials of semiscale
production of PHAs in 14 L laboratory fermentor cultures.
Different fermentation techniques were applied in order
to increase the PHAs yield using WFO as the sole
carbon source.
One-stage batch culture
In batch culture, the cell mass increased gradually
leading to record the maximum values of cell dry weight
and polymer concentration (2.93 and 0.92 g/L, respectively)
after 72 h of cultivation (Table 1). Whereas, the
maximum PHAs content (33.7%) was obtained after 60 h