In most cases of in vitro
selection for salinity, selection was either applied to
explants or cell/callus culture by inclusion of growth
inhibitory levels of salt in the selection medium
(Suprasanna and Rao 1997). To study the salt inhibitory
level, the nonirradiated callus was exposed to different
NaCl concentrations and the results indicated
regeneration of only up to 171.1 mM selections. The
survival response and the growth of calli also showed a
decreasing trend with an increasing concentration of
salt. Therefore in studies on in vitro mutagenesis and
selection, the media were designed accordingly